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Symmetry: Culture and Science
Volume 32, Number 2, pages 193-196 (2021)
https://doi.org/10.26830/symmetry_2021_2_193

CUTTING EDGE SCIENCE REVEALS THE SECRETS OF CHIROMORPHOGENESIS IN SNAILS

Reiko Kuroda*

* Frontier Research Institute, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan
E-mail: rkuroda@isc.chubu.ac.jp
ORCID: 0000-0002-0268-7729

Abstract: Chiromorphology is a field of research focusing on the molecular origins and the molecular mechanisms that lead to chiral macroscopic objects and phenomena. Here we describe twenty years of our chiromorphology research on the pond snail Lymnaea stagnalis to discover symmetry breaking during chiromorphogenesis, to create situs inversus snails mechanically, to identify the single gene that determines body handedness, and to create situs inversus snails by using the cutting-edge science of CRISPR genome editing of the candidate gene.

References:
Abe, M., Kuroda, R. (2019) The development of CRISPR for a mollusc establishes the formin Lsdia1 as the long-sought gene for snail dextral/sinistral coiling. Development, 146, 9, dev175976. https://doi.org/10.1242/dev.175976

Boycott, A.E., Diver, C. (1923) On the inheritance of sinistrality in Lymnaea peregra. Proc. R. Soc. Biol. Sci. Ser. B, 95, 207-213. https://doi.org/10.1098/rspb.1923.0033

Davison, A., McDowell, G.S., Holden, J.M., Johnson, H.F., Koutsovoulos, G.D., Liu, M.M., Hulpiau, P., Van Roy, F., Wade, C.M., Banerjee, R. et al. (2016) Formin is associated with left-right asymmetry in the pond snail and the frog. Curr. Biol., 26, 654-660. https://doi.org/10.1016/j.cub.2015.12.071

Hosoiri, Y., Harada, Y., Kuroda, R. (2003) Construction of a backcross progeny collection of dextral and sinistral individuals of a freshwater gastropod, Lymnaea stagnalis. Dev. Genes Evol., 213, 4, 193-198. https://doi.org/10.1007/s00427-003-0315-y

Kuroda, R. (2014) How a single gene twists a snail. Integr. Comp. Biol., 54, 4, 677-687. https://doi.org/10.1093/icb/icu096

Kuroda, R. (2015) A twisting story: how a single gene twists a snail? Mechanogenetics. Q. Rev. Biophys., 48, 445-452. https://doi.10.1017/S0033583515000098

Kuroda, R., Mason S.F. (1981) Crystal structure of dextrorotatory and racemic sodium ammonium tartrate. J. Chem. Soc. Dalton Trans., 1268-1273. https://doi.org/10.1039/DT9810001268

Kuroda, R., Endo, B., Abe, M., Shimizu M. (2009) Chiral blastomere arrangement dictates zygotic left-right asymmetry pathway in snails. Nature, 462, 790-794. https://doi.org/10.1038/nature08597

Kuroda, R., Fujikura, K., Abe, M., Hosoiri, Y., Asakawa, S., Shimizu, M., Umeda, S., Ichikawa, F., Takahashi, H. (2016) Diaphanous gene mutation affects spiral cleavage and chirality in snails. Sci. Rep., 6, 34809. https://doi.org/10.1038/srep34809

Kuroda Chiromorphology Project. (1999) ERATO, Japan Science and Technology Corporation.

Shibazaki, Y., Shimizu M., Kuroda R. (2004) Body handedness is directed by genetically determined cytoskeletal dynamics in the early embryo. Curr. Biol., 14, 1462-1467. https://doi.10.1016/j.cub.2004.08.018

Sturtevant, A.H. (1923) Inheritance of direction of coiling in Lymnaea. Science, 58, 269-270. https://doi.10.1126/science.58.1501.269

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